Protein_Domain

Part:BBa_K1362470

Designed by: Jakob Kreft   Group: iGEM14_Heidelberg   (2014-10-09)


sfGFP_T65C sequence (Mutated BBa_I746917)

This is a mutated version of superfolder GFP BBa_I746917 the T at position 65 has been mutated to a C. This mutation was inserted to serve as a control for the intein splicing reaction tested with the iGEM team Heidelberg 2014's split fluorescent protein assay.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 10


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Categories
Parameters
None